Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick standard vector
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K864004_sequence (Version 1) |
Description
pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
Source
based on pSB3T5| Sequence Annotation | Location | Component / Role(s) |
| Lambda Red reverse primer TonB terminator VR rrnC terminator pSC101 ori BBa_K864050 Lambda Red forward primer Lux terminator P22 late terminator VF2 T3 terminator AmpR P1002 RBS -10 -35 FRT site FRT site | 18,38 47,115 116,135 136,175 190,2405 2433,2450 2454,2499 3523,3563 3564,3583 3584,3661 2543,3403 3408,3412 3421,3426 3447,3452 2506,2539 3483,3516 | primer_binding_site feature/primer_binding stem_loop feature/stem_loop primer_binding_site feature/primer_binding feature/stem_loop stem_loop feature/misc sequence_feature feature/primer_binding primer_binding_site feature/stem_loop stem_loop stem_loop feature/stem_loop feature/primer_binding primer_binding_site stem_loop feature/stem_loop CDS feature/cds feature/rbs ribosome_entry_site feature/promoter promoter promoter feature/promoter sequence_feature feature/misc sequence_feature feature/misc |