BBa_K864120

BBa_K864120 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K864120

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Arvid Hed??n Gynn??

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:37

pMB1 ori - RFP cassette

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K864120_sequence (Version 1)

Description

__NOTOC__
BBa_K864121 short

This is a minimal high-copy replication origin coupled to the standard RFP casette J04450. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pMB1ori-red".

LacI sensitive
CAP sensitive

Usage and Biology

Usable as default insert of low and medium copy backbones. This is the default insert of the pSB4x15 series of backbones.

Sequence and Features
BBa_K864121 SequenceAndFeatures

Notes

Assembled for use in low and medium copy backbones.

Source

The pMB1 origin has been PCR amplified from pSB1A3.

Components

Access	Instance	Definition
public	BBa_I50022	pUC19 ori
public	BBa_J04450	BBa_J04450

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I50022	1,674	pUC19 ori
BBa_J04450	683,1751	BBa_J04450

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K864120/1

Member of these Collections

• iGEM Parts Registry