redq

BBa_K864502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K864502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arvid Hed??n Gynn??
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:37

RFP Coding Device, constitutive



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K864502_sequence (Version 1)

Description

Similar to the commonly used RFP cassette [[BBa_J04450]], but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones.

When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.

Notes

The CP44 promoter was chosen to be similar to the LacI promoter.

Source

A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K864502/1