Types | DnaRegion
|
Roles | Reporter
engineered_region
|
Sequences | BBa_K864502_sequence (Version 1)
|
Description
Similar to the commonly used RFP cassette [[BBa_J04450]], but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones.
When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.
Notes
The CP44 promoter was chosen to be similar to the LacI promoter.
Source
A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.