Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Mungu??a D??az
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:37
CO2 biosensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K872001_sequence (Version 1) |
Description
This compose part forms a system for CO2 biosensing based in two modules, the first module (module I) resembles a ???signal converter??? and the second module (module II) functions as a ???output generator???.The first module is compose by the mild promoter J23101 with rbs B0012 attached to protein sAC and terminator B0030. Protein sAC (for soluble Adenilate Cyclase) is a 854 bp long and its function is to generate cAMP form ATP based on HCO3- concentration. This protein is involved in pH sensing, CO2 sensing and even in spermatozoa capacitance.
The second module is TU_Delft 2010 biobrick BBa_K398331 composed by cAMP-CRP inducible promoter pCaif with rbs B0032 attached to GFP and dual terminator B0010 and B0012
The system works as follow. CO2 diffuse into water and reaction with water (H2O) by carbonic anhydrase enzyme forming HCO3- and H+. HCO3- accumulates inside bacteria and activates sAC producing cAMP as and intermediate signaling molecule, cAMP activates CRP protein and binds in pCaif promoter activating the formation of GFP. This allowed us to measure the CO2 concentration (indirectly by the formation of bicarbonate ion) as a function of GFP. The next diagram illustrate the system.
Notes
BamHI restriction site added, this is a very sensitive biosensorSource
For module I secuence can be found in http://partsregistry.org/Part:BBa_K872000For module II TU_Delft 2010 biobrick secuence can be found in http://partsregistry.org/Part:BBa_K398331
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 RBS-3\Weak BBa_B0032 RBS BBa_J23101 RBS-3\Weak BBa_B0032 RBS BBa_J23101 BBa_B0032 RBS-3\Weak RBS BBa_K872000 BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop Crp-cAMP BBa_K398326 BBa_B0032 RBS-3\Weak RBS BBa_E0040 GFP protein BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_K398331 | 1,35 44,56 44,56 50,53 65,99 108,120 108,120 114,117 129,163 172,184 172,184 178,181 191,1047 1056,1184 1056,1135 1067,1110 1144,1184 1151,1170 1171,1184 1177,1177 1193,1321 1193,1272 1204,1247 1281,1321 1288,1307 1308,1321 1314,1314 1330,1409 1330,1458 1341,1384 1418,1458 1425,1444 1445,1458 1451,1451 1467,1487 1467,1517 1526,1538 1526,1538 1532,1535 1545,2264 1545,2264 2273,2352 2284,2327 2361,2401 2368,2387 2388,2401 2394,2394 1467,2401 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/rbs ribosome_entry_site engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site stop_codon feature/stop non_covalent_binding_site feature/binding engineered_region feature/BioBrick feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/protein CDS engineered_region feature/BioBrick feature/stem_loop stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region |