Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Josh Atkinson, Mike Ferguson, and Ben Parker
Date created: 2012-09-28 11:00:00
Date modified: 2015-05-08 01:13:40
GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K880002_sequence (Version 1) |
Description
-IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275)-GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. Same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - ???flip region??? - IRL)
GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058.
K137058???s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have ???left??? and ???right??? components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order ???K137008-region to invert-K137010??? in accordance with the wild-type fim system.
Repeat sequence in this part exist in the correct (wild-type) orientation.
Notes
Enter design considerationsSource
Enter source| Access | Instance | Definition |
| public public public public | BBa_K137008 BBa_K137047 BBa_K137010 BBa_I13504 | IRR BBa_K137047 fimE IRL BBa_I13504 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K137008 BBa_K137047 BBa_K137010 BBa_I13504 | 1,35 44,293 302,336 345,1219 | IRR BBa_K137047 fimE IRL BBa_I13504 |