Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chia-Jung Lee
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:40
Ptet(promoter)+B0032(RBS)+Gli1(zinc finger)+kivD(enzyme)+B0015(terminator)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K887051_sequence (Version 1) |
Description
This is the coding sequence of a fusion protein which contain kivd from Lactococcus lactis subsp., linked with a zinc finger Gli1. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination.Kivd plays a vital rule in the isobutanol biosynthesis pathway. And the zinc finger can help us fixing enzyme on the DNA program to assemble a production line.Notes
34Source
12| Access | Instance | Definition |
| public public public public public | BBa_R0040 BBa_B0032 BBa_K165007 BBa_K539714 BBa_B0015 | p(tetR) BBa_B0032 BBa_K165007 BBa_K539714 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_B0032 BBa_K165007 BBa_K539714 BBa_B0015 | 1,54 63,75 84,608 615,2261 2270,2398 | p(tetR) BBa_B0032 BBa_K165007 BBa_K539714 BBa_B0015 |