Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hsin-Hui Ling
Date created: 2012-04-26 11:00:00
Date modified: 2015-05-08 01:13:42
Mediate Cellular Resistance
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K896919_sequence (Version 1) |
Description
The human 1-8 interferon inducible gene family consists of at least 3 functional genes; 9-27, 1-8D and 1-8U, which are all linked on an 18-kb fragment of chromosome 11 and are highly homologous. It has recently been shown by us and others that the 1-8D gene is overexpressed in colon carcinoma. Here, we show, by sequence comparison of the 1-8D in pairs of tumor/normal colon tissues, the existence of 6 different alleles, containing single-nucleotide polymorphisms with no mutations. Transformation assays revealed a possible role for the 1-8D gene as a transformation inhibitor. Further, transient expression of the human 1-8D gene in multiple mammalian cell lines showed accumulation of cells in the G1 phase followed by elevation in the subG1 phase. SubG1 elevation was confirmed as apoptosis by Annexin-V binding assays and transferase-mediated dUTP nick end labeling assays. Moreover, knock-down of 1-8D provided partial protection from Etoposide and UV-induced apoptosis. The induction of apoptosis by 1-8D is dependent on caspase activities but not on p53 expression. Although 1-8D induces apoptosis independently of p53, p53 expression downregulates 1-8D protein expression. Our data suggest a role for the 1-8D gene as a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.Notes
duration is short.Source
Sus scrofa| Access | Instance | Definition |
| public public public public | BBa_I712004 BBa_B0034 BBa_B0010 BBa_B0012 | BBa_I712004 BBa_B0034 BBa_B0010 BBa_B0012 |