BBa_K906102

BBa_K906102 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K906102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lucas B. Harrington

Date created: 2012-08-21 11:00:00

Date modified: 2015-05-08 01:13:44

ZCD with Histidine Patch Thioredoxins and Hist-tag

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K906102_sequence (Version 1)

Description

This is the functional unit which makes ZCD soluble in E. coli. It includes a inducible promoter, mutated thioredoxin to increase solubility, the ZCD ORF, a hist-tag for easy purification and finally a double terminator. This part has been shown to highly express ZCD in E. coli. It is suggested that this part be used in a plasmid also containing araC for the optimal repression of this protein before the time of induction.

Notes

This part was codon optimized for Synechocystis, reflecting its planned expression in this cyanobacteria.

Source

ZCD originated from Crocus Sativus, the saffron plant. However the version present here has been codon optimized for synechocystis.

Components

Access	Instance	Definition
public	BBa_K906007	BBa_K906007
public	BBa_K906003	BBa_K906003
public	BBa_K906000	BBa_K906000
public	BBa_K906004	BBa_K906004
public	BBa_K906005	BBa_K906005

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K906007	1,345	BBa_K906007
BBa_K906003	346,678	BBa_K906003
BBa_K906000	679,1788	BBa_K906000
BBa_K906004	1789,1875	BBa_K906004
BBa_K906005	1876,2135	BBa_K906005

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K906102/1

Member of these Collections

• iGEM Parts Registry