Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lucas B. Harrington
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:44
ZCD with Histidine Patch Thioredoxins and Hist-tag
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K906102_sequence (Version 1) |
Description
This is the functional unit which makes ZCD soluble in E. coli. It includes a inducible promoter, mutated thioredoxin to increase solubility, the ZCD ORF, a hist-tag for easy purification and finally a double terminator. This part has been shown to highly express ZCD in E. coli. It is suggested that this part be used in a plasmid also containing araC for the optimal repression of this protein before the time of induction.Notes
This part was codon optimized for Synechocystis, reflecting its planned expression in this cyanobacteria.Source
ZCD originated from Crocus Sativus, the saffron plant. However the version present here has been codon optimized for synechocystis.| Access | Instance | Definition |
| public public public public public | BBa_K906007 BBa_K906003 BBa_K906000 BBa_K906004 BBa_K906005 | BBa_K906007 BBa_K906003 BBa_K906000 BBa_K906004 BBa_K906005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K906007 BBa_K906003 BBa_K906000 BBa_K906004 BBa_K906005 | 1,345 346,678 679,1788 1789,1875 1876,2135 | BBa_K906007 BBa_K906003 BBa_K906000 BBa_K906004 BBa_K906005 |