Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fai Tsang
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:45
Fluorescent ratiometric construct for standardizing promoter output
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K911009_sequence (Version 1) |
Description
Biosensors may give unreliable outputs. This is due to differences in the number and state of the cells from test to test. By including an internal control signal, to which another inducible signal may be normalised, the reliability and reproducibility of a sensor may be significantly improved.The construct that uses an inducible eCFP (E0020) and a constitutively expressed eYFP (E0030) under the control of the constitutive promoter Pveg (K143012). The construct is optimized in both E. coli and B. subtilis, through the use of B. subtilis ribosome binding sites GsiB (K143020) and SpoVG (K143021).
Notes
This biobrick is best used with a shuttle vector that can function in both E. coli and B. subtilis, which should also have preferably different antibiotic markers in E. coli and B. subtilis.Source
This biobrick is composed entirely of existing biobricks: GsiB (K143020), eCFP (E0020), terminator (B0015), Pveg + SpoVG (K143053), eYFP (E0030) and a second terminator (B0010). It was constructed first on the E. coli- B. subitilis shuttle vector pJS130, then moved onto pSB1C3 for submission.| Access | Instance | Definition |
| public public public public public public | BBa_K143020 BBa_E0020 BBa_B0015 BBa_K143053 BBa_E0030 BBa_B0010 | RBS-GsiB ecfp BBa_B0015 Pveg-spoVG eyfp BBa_B0010 |