Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elitsa Peeva
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46
lac promoter plus sucrose hydrolase (cscA)
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K917011_sequence (Version 1) |
Description
This is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene under the regulation of the lac promoter. K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence).Notes
Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included.Source
Escherichia coli O157:H7 strain Sakai| Access | Instance | Definition |
| public public | BBa_J33207 BBa_K917000 | BBa_J33207 BBa_K917000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_K917000 | 1,600 609,2052 | BBa_J33207 BBa_K917000 |