Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46
Anti-EGFR scFv425 with TEV site, transmembrane region an eYFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K929101_sequence (Version 1) |
Description
The Biobrick BBa_K929101 is an extended version of the Biobrick BBa_K929100 and is composed of the already existing human EGFR (ErbB-1) signal peptide (BBa_K157001), the scFv anti-EGFR-domain-3, a TEV recognition site (according to life technologies AcTEV TM Protease manual), the transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010) and the enhanced YFP ( BBa_E0030) reporter. The composite part was derived by PCR assembly.Notes
The composite part was derived by PCR assembly.Source
BBa_K157001, BBa_K929100, BBa_K157010, BBa_E0030| Sequence Annotation | Location | Component / Role(s) |
| SP ErbB-1 Signal peptide BBa_K157001 Flag tag scFv425 BBa_K929100 GGGGSx3-BCR-Transmembrane BBa_K157010 BBa_E0030 TEV recognition site | 1,72 1,72 1,72 81,93 94,821 81,821 853,975 830,937 944,1680 822,852 | feature/protein CDS feature/cds CDS engineered_region feature/BioBrick sequence_feature feature/misc CDS feature/cds feature/BioBrick engineered_region feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region feature/misc sequence_feature |