Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:47
Anti-GFP, IgG1 Fc with TEV site, TMD, mCherry, framed by LoxP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K929107_sequence (Version 1) |
Description
The antibody construct consists of two major building blocks represented by the actual antibody unit ( BBa_K929102) and the switchable membrane anchoring region (BBa_K929103). Both elements guarantee the eligibility and the easy handling of the construct and are optimized for expression in CHO cells. The antibody unit is represented by the human Ig kappa chain V-I region signal peptide (UniProt: P01601), the anti-GFP Nanobody (PDB: 3OGO) and the Fc region (UniProt: P01857). TEV protease recognition site, 2 LoxP sites, the B-cell receptor transmembrane domain (modified BBa_K157010) and the mcherry reporter display the switchable membrane anchoring region.Notes
[1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82[2] Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401
Source
BBa_K929102 and BBa_K929103| Sequence Annotation | Location | Component / Role(s) |
| IgG kappa signal peptide BBa_K929102 anti-GFP nanobody IgG1 Fc domain BBa_K929103 TEV recognition site LoxP site 1 B-cell receptor transmembrane domaine with linker mCherry misc | 1,66 1,1116 67,414 421,1116 1117,2019 1123,1143 1144,1177 1180,1254 1267,1974 1978,2011 | feature/misc sequence_feature feature/BioBrick engineered_region feature/cds CDS feature/cds CDS engineered_region feature/BioBrick sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc CDS feature/cds feature/misc sequence_feature |