Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Justin Emlen
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:47
An L-Arabinose Controlled Vitamin B12 Binding - Starch Binding Protein Fusion
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K931003_sequence (Version 1) |
Description
L-arabinose induced Starch Binding-B12 Binding Protein is a functional construct which contains the BtuF gene that encodes for a B12 binding protein and a starch binding domain encoded by the gene, CBM 21. The construct is under the control of an L-arabinose promoter (BBa_K206000) and a terminator (BBa_K206000). The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli???s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively.Notes
N/ASource
Multiple| Access | Instance | Definition |
| public public public public public | BBa_K206000 BBa_B0034 BBa_K931001 BBa_K931000 BBa_B0015 | pBAD BBa_B0034 BBa_K931001 BBa_K931000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K931001 BBa_K931000 BBa_B0015 | 1,130 139,150 157,954 963,1324 1333,1461 | pBAD BBa_B0034 BBa_K931001 BBa_K931000 BBa_B0015 |