Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jasmine Jiang
Date created: 2012-09-27 11:00:00
Date modified: 2015-05-08 01:13:47
SBP - B12BP
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K931007_sequence (Version 1) |
Description
A functional construct which contains the BtuF gene that ecodes for a B12 binding protein and a starch binding domain encoded by the gene, CBM 21. The construct is under the control of an L-arabinose promoter (BBa_K206000) and a terminator (BBa_K206000). The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli???s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively.Notes
N/ASource
This composite part comes from conjugating the gene BtuF from E. coli and CBM 21 from R. oryzae| Access | Instance | Definition |
| public public | BBa_K931000 BBa_K931001 | BBa_K931000 BBa_K931001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K931000 BBa_K931001 | 1,362 369,1166 | BBa_K931000 BBa_K931001 |