BBa_K932003

BBa_K932003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K932003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sean Kearney

Date created: 2012-10-10 11:00:00

Date modified: 2015-05-08 01:13:47

OmpA-Silicatein-Alpha under Lac promoter

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K932003_sequence (Version 1)

Description

This part includes a silica-nucleating outer membrane protein under the control of the lac repressible promoter. Induction with IPTG results in the expression of protein with the tet repressor for repression of heterologous DNA constructs.

Notes

The design is intended to be inducible only in the presence of lactose analogs.

Source

OmpA is from E. coli K-12
Silicatein-alpha truncated sequence is from Tethya aurantia.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K932002	BBa_K932002
public	BBa_B0034	BBa_B0034
public	BBa_C0040	tetR
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K932002	227,1344	BBa_K932002
BBa_B0034	1353,1364	BBa_B0034
BBa_C0040	1371,2055	tetR
BBa_B0014	2064,2158	BBa_B0014

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K932003/1

Member of these Collections

• iGEM Parts Registry