Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hannah Jepsen-Burger
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:47
two start codons coding for RFP and sfGFP reporters
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K933000_sequence (Version 1) |
Description
This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.Notes
This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct.Source
This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments.| Sequence Annotation | Location | Component / Role(s) |
| weak RBS start codon RFP (ORF 0) start codon sfGFP (ORF +1) 41 1N break in reference frame sfGFP reporter double terminator | 1,28 29,30 39,40 41,709 710,710 711,1427 1461,1589 | feature/rbs ribosome_entry_site start_codon feature/start feature/start start_codon feature/protein CDS feature/stop stop_codon CDS feature/protein stem_loop feature/stem_loop |