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BBa_K945005

BBa_K945005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K945005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Villalobos Ram??rez

Date created: 2012-09-26 11:00:00

Date modified: 2015-05-08 01:13:48

Pichia pastoris optimized His-GFP-PTS1

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_K945005_sequence (Version 1)

Description

This sequence codes for a green fluorescent protein (GFP) which has been codon optimized for Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (TPS1) in its C-terminus.

Notes

During codon optimization, restriction sites inside the coding sequence had to be avoided for it to be compatible with assembly standards.

Source

This sequence is a modification and optimization of the original wild-type GFP found in Aequorea victoria.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
wt-GFP AOX1 terminator 6xHis-tag PTS1 Yeast RBS	39,776 777,1117 42,59 765,773 26,38	feature/protein CDS feature/misc sequence_feature tag feature/tag tag feature/tag ribosome_entry_site feature/rbs