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BBa_K945006

BBa_K945006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K945006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Villalobos Ram??rez

Date created: 2012-09-26 11:00:00

Date modified: 2015-05-08 01:13:48

Pichia pastoris optimized His-CrtE-PTS1

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K945006_sequence (Version 1)

Description

This sequence codes for CrtE (GGPP synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.

Notes

The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.

Source

This sequence is originally derived from the CDS for geranylgeranyl pyrophosphate synthase from Pantoea ananatis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CrtE Yeast RBS 6xHis-tag PTS1 AOX1 terminator	20,982 8,19 23,40 971,979 983,1323	feature/protein CDS ribosome_entry_site feature/rbs feature/tag tag feature/tag tag sequence_feature feature/misc