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BBa_K945007

BBa_K945007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K945007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Villalobos Ram??rez

Date created: 2012-09-26 11:00:00

Date modified: 2015-05-08 01:13:48

Pichia pastoris optimized His-CrtB-PTS1

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K945007_sequence (Version 1)

Description

This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.

Notes

The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.

Source

This sequence is originally derived from the CDS for phytoene synthase from Pantoea ananatis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CrtB Yeast RBS AOX1 Terminator 6xHis-tag PTS1	41,997 29,40 998,1338 44,61 986,994	feature/protein CDS ribosome_entry_site feature/rbs sequence_feature feature/misc feature/tag tag feature/tag tag