BBa_M11207

BBa_M11207 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M11207
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angela Dixon
Date created: 2010-04-21 11:00:00
Date modified: 2015-05-08 01:13:53

Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_M11207_sequence (Version 1)

Description

Used in S. cerevisiae to monitor glucose and oxygen levels. It is composed of two BioBrick devices:

Device 1:
The promoter, HXT7, is repressed in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Device 2:
The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Notes

None.

Source

Composite of the following BioBrick devices: BBa_M11204, BBa_M11206

Access Instance Definition
public
public
BBa_M11204
BBa_M11206
BBa_M11204
BBa_M11206
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_M11207/1