Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angela Dixon
Date created: 2010-04-21 11:00:00
Date modified: 2015-05-08 01:13:53
Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_M11208_sequence (Version 1) |
Description
Used in S. cerevisiae to monitor glucose and oxygen levels. It is composed of two BioBrick devices:Device 1:
The promoter, HXT1, is activated in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
Device 2:
The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
Notes
None.Source
Composite of the following BioBrick devices: BBa_M11205, BBa_M11206| Access | Instance | Definition |
| public public | BBa_M11205 BBa_M11206 | BBa_M11205 BBa_M11206 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M11205 BBa_M11206 | 1,2691 2700,3759 | BBa_M11205 BBa_M11206 |