Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2010-04-23 11:00:00
Date modified: 2015-05-08 01:13:53
Integration Plasmid for Synechocystis sp. PCC 6803
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_M11400_sequence (Version 1) |
Description
This plasmid is designed to integrated into the Synechocystis sp. PCC 6803 genome. It contains an ampicillin resistance gene and an E. coli origin of replication, so BioBrick parts can be assembled in E. coli, then transformed into Synechocystis for genomic integration. In Synechocystis, transformed cells can be selected with kanamycin. M11400 is based on plasmid pIGA from Kunert et al. (2000), GenBank accession number AJ251839.1.The recombination sites correspond to the regions containing hypothetical proteins slr0338 and slr0168 in Synechocystis sp. PCC 6803.
Notes
Site-directed mutagenesis of EcoRI site between RS1 and kanamycin resistance gene. Removal of GFP expression cassette and T7 terminator. Addition of translation and transcription stop sequences flanking BioBrick prefix and suffix sites (from BioBrick Basic Vector BBa_I51020).Source
Genomic Sequence, Genbank| Sequence Annotation | Location | Component / Role(s) |
| Kanamycin Resistance (aphII) Start Stop RS2 (recombination region for Synechocystis sp PCC 6803 - region of hypothetical gene slr0168) RS1 (recombination region for Synechocystis PCC 6803 - region of hypothetical gene slr0338) Ampicillin Resistance (bla) PstI Site BBa_B0042 OriV dnaA box EcoRI Site XbaI Site SpeI Site BBa_B0042 BBa_B0053 BBa_B0054 BBa_B0055 Start BBa_B0062 Stop BBa_G00000 BBa_G00001 BBa_G00100 (VF2) BBa_G00102 (VR) | 1,816 1,3 814,816 1554,2922 5272,6951 3532,4392 1292,1297 1267,1278 3131,3140 1336,1341 1321,1326 1306,1311 1355,1366 1465,1536 1198,1266 1367,1444 3532,3534 1137,1177 4390,4392 1321,1341 1292,1311 1446,1464 1178,1197 | CDS feature/protein feature/start start_codon stop_codon feature/stop sequence_feature feature/dna sequence_feature feature/dna CDS feature/protein sequence_feature feature/misc feature/BioBrick engineered_region feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region start_codon feature/start engineered_region feature/BioBrick feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding |