BBa_M11522

BBa_M11522 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M11522

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alyssa Calder

Date created: 2010-04-19 11:00:00

Date modified: 2015-05-08 01:13:54

Zinc Promoter (ZntR regulated) with RFP Reporter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_M11522_sequence (Version 1)

Description

Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound (1) and produces RFP engineered mutant of red fluorescent protein from Discosoma striata (coral).

Notes

Composite part of BBa_K190016 and BBa_E1010

Source

1) C.E. Outten, F.W. Outten, T.V. O???Halloran (1999) DNA Distortion Mechanism for Transcriptional Activation by ZntR, a Zn(II)-responsive MerR Homologue in Escherichia coli, The Journal of Biological Chemistry, Vol. 274, No. 53, Issue of December 31, pp. 37517???37524.

Components

Access	Instance	Definition
public	BBa_K190016	BBa_K190016
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K190016	1,69	BBa_K190016
BBa_B0034	78,89	BBa_B0034
BBa_E1010	96,801	mRFP1
BBa_B0010	810,889	BBa_B0010
BBa_B0012	898,938	BBa_B0012

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M11522/1

Member of these Collections

• iGEM Parts Registry