Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Allred
Date created: 2012-04-17 11:00:00
Date modified: 2015-05-08 01:13:56
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M1533_sequence (Version 1) |
Description
This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.Notes
See subpartsSource
See subparts| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_K208010 HlyA Start BBa_K208006 conserved BBa_B0034 start cds tag stop BBa_M1525 BBa_B0035 Start Codon HlyB BBa_M1003 Stop Codon BBa_B0035 Start Codon HlyD BBa_M1004 Stop Codon BBa_M1528 conserved BBa_B0034 HlyA Start BBa_K208006 conserved BBa_B0034 Start GFP Coding Region BBa_K208000 BBa_B0035 Start Codon HlyB BBa_M1003 Stop Codon BBa_B0035 Start Codon HlyD BBa_M1004 Stop Codon BBa_M1528 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,200 209,220 1,220 229,414 229,231 229,414 427,430 423,434 441,443 444,920 921,944 945,947 441,947 956,969 976,978 976,3096 976,3099 3097,3099 3108,3121 3128,3130 3128,4561 3128,4564 4562,4564 956,4564 4577,4580 4573,4584 4593,4778 4593,4595 4593,4778 4791,4794 4787,4798 4805,4807 4805,5524 4805,5524 5533,5546 5553,5555 5553,7673 5553,7676 7674,7676 7685,7698 7705,7707 7705,9138 7705,9141 9139,9141 5533,9141 9150,9229 9161,9204 9238,9278 9245,9264 9265,9278 9271,9271 9150,9278 | promoter feature/promoter feature/rbs ribosome_entry_site engineered_region feature/BioBrick tag feature/tag start_codon feature/start engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/start start_codon feature/cds CDS feature/tag tag stop_codon feature/stop engineered_region feature/BioBrick engineered_region feature/BioBrick feature/start start_codon CDS feature/cds engineered_region feature/BioBrick feature/stop stop_codon engineered_region feature/BioBrick feature/start start_codon CDS feature/cds engineered_region feature/BioBrick feature/stop stop_codon feature/BioBrick engineered_region feature/conserved conserved_region feature/BioBrick engineered_region tag feature/tag feature/start start_codon feature/BioBrick engineered_region feature/conserved conserved_region engineered_region feature/BioBrick start_codon feature/start CDS feature/protein engineered_region feature/BioBrick engineered_region feature/BioBrick start_codon feature/start CDS feature/cds engineered_region feature/BioBrick feature/stop stop_codon feature/BioBrick engineered_region start_codon feature/start feature/cds CDS feature/BioBrick engineered_region feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region |