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BBa_M30002

BBa_M30002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M30002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: natalie kuldell

Date created: 2005-11-24 12:00:00

Date modified: 2015-05-08 01:13:58

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_M30002_sequence (Version 1)

Description

P22 c2 inverter (Q04530) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04530 fragment with four-part inverter. Cells carrying M30002 are red, however because PCR was used for cloning there may be silent mutations in the insert. M30002 is clone #2. A second clone will be saved as M30001.

Components

Access	Instance	Definition
public public public public public public public public public	BBa_B0034 BBa_C0053 BBa_B0010 BBa_B0012 BBa_R0053 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	BBa_B0034 c2 P22 BBa_B0010 BBa_B0012 cII p22 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034 BBa_C0053 BBa_B0010 BBa_B0012 BBa_R0053 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	1,12 19,705 739,818 827,867 876,929 938,949 956,1636 1670,1749 1758,1798	BBa_B0034 c2 P22 BBa_B0010 BBa_B0012 cII p22 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012