Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matt Gethers
Date created: 2007-02-28 12:00:00
Date modified: 2015-05-08 01:14:00
Refactored Portion of M13K07 Genome
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M31530_sequence (Version 1) |
Description
I've refactored the segment of DNA between the unique HapI and BamHI sites. Following the guidlines in "Refactoring T7" by Chan et al, I've determined what functional components that I would like to be physically separated from one another (namely regulatory regions and ORFs)and set them apart in my design. I'm not sure that these modifications will yield a functional phage. Even viable phage result, I don't know what sort of emergent functions I will have created by refactoring the genome.Notes
In order to organize the M13K07 genome into individual components, some sequences must be "copied and pasted" elsewhere. This opens the possibility for recomination events or coding regions that code for functions for which they are no longer responsible (legacy functions). These interactions must be addressed to avoid interactions bewteen stretches of DNA. As far as I know, this can be addressed by changing codon usage, introducing inhibitory secondary structures to the DNA, or by eliminating the enzymatic mechanisms by which these events may occur. I'm sure there are others. Another consideration is that absolute and relative position in the genome may be of importance. I will try to maintain the "localities" as best I can so as not to introduce further sources of problems.Source
This part does not yet exist in a physical form, though it may be synthesized in the near future.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public | BBa_M13102 BBa_M13502 BBa_M13002 BBa_M13110 BBa_M13510 BBa_M13010 BBa_M13105 BBa_M13505 BBa_M13005 BBa_M13507 BBa_M13007 BBa_M13509 BBa_M13009 BBa_M13108 BBa_M13508 BBa_M13008 BBa_M13103 BBa_M13503 BBa_M13003 | BBa_M13102 BBa_M13502 BBa_M13002 BBa_M13110 BBa_M13510 BBa_M13010 BBa_M13105 BBa_M13505 BBa_M13005 BBa_M13507 BBa_M13007 BBa_M13509 BBa_M13009 BBa_M13108 BBa_M13508 BBa_M13008 BBa_M13103 BBa_M13503 BBa_M13003 |