Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tiffany Guo
Date created: 2007-02-28 12:00:00
Date modified: 2015-05-08 01:14:01
M13 refactoring
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M31975_sequence (Version 1) |
Description
M13K07 refactored to elimiate overlaps and add restriction enzyme sites inbetween parts.Notes
1) eliminate rbs and promoters within genes: took into consideration codon changes that would not change amino acid sequences but would decrease the strength of the rbs or promoter.2) add restriction sites between each part: tried to keep sticky ends as consistent as possible (i.e. after every promoter, the restriction site sticky end is 3'ctag), however, there aren't enough zero cutting isoschizomers.
Source
parts from M13K07 genome| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public public | BBa_M13002 BBa_M31997 BBa_M13110 BBa_M31994 BBa_M13510 BBa_M31990 BBa_M13010 BBa_M31996 BBa_M13105 BBa_M31992 BBa_M13505 BBa_M31989 BBa_M31981 BBa_M31995 BBa_M13507 BBa_M31988 BBa_M31980 BBa_M31987 BBa_M13509 BBa_M31986 BBa_M31978 BBa_M31985 BBa_M13108 BBa_M31993 BBa_M13508 BBa_M31984 BBa_M13008 BBa_M31983 BBa_M31977 BBa_M31976 BBa_M13103 BBa_M31991 BBa_M13503 BBa_M31982 BBa_M13003 | BBa_M13002 Acc651 BBa_M13110 AvrII BBa_M13510 AscI BBa_M13010 BsiWI BBa_M13105 SpeI BBa_M13505 BssHII BBa_M31981 BsrG1 BBa_M13507 MluI BBa_M31980 BclI BBa_M13509 EagI BBa_M31978 BglII BBa_M13108 NheI BBa_M13508 PspOMI BBa_M13008 EcoRI BBa_M31977 BBa_M31976 BBa_M13103 XbaI BBa_M13503 NotI BBa_M13003 |