Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Virrueta
Date created: 2010-06-05 11:00:00
Date modified: 2015-05-08 01:14:02
RBS+CDS for TodT protein
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M33354_sequence (Version 1) |
Description
Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed pTod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodT is phosphorylated by the protein kinase TodS, and it can also increase the autophosphorylation activity of TodS. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription.This part should be used in conjunction with BBa_M33351, the Ptod promoter, and BBa_M33350/BBa_M33352, the todS protein sequence.
Notes
Made 1 silent mutation for XbaI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA, and added a strong ribosome binding site (BBa_B0034).Source
This is a genomic sequence from Pseudomonas putida. The was obtained from GenBank at http://www.ncbi.nlm.nih.gov/nuccore/4914628 (Y18245.1; todT coordinates: 14162..14845).| Access | Instance | Definition |
| public public | BBa_B0034 BBa_M33353 | BBa_B0034 BBa_M33353 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_M33353 | 1,12 19,702 | BBa_B0034 BBa_M33353 |