Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Shih
Date created: 2013-04-22 11:00:00
Date modified: 2015-05-08 01:14:03
E. coli sensor test rig
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_M36099_sequence (Version 1) |
Description
E. coli test vector for sensors. Has a Gemini protein coding sequence which produces a LacZalpha-GFP fusion protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.Notes
NoneSource
Gemini coding sequence sourced from Dr. Drew Endy and Martin et al., PlosOne 2009. The rest of the vector is sourced from DNA2.0.| Sequence Annotation | Location | Component / Role(s) |
| ampR pUCori rpo_bla transcription terminator student sensors go here RBS Comet GFP T7 terminator rrnB1T1T2 transcripton terminator cloning cassette 1 cloning cassette 2 Pamp | 1,861 900,1704 1705,2313 2373,2434 2435,2448 2449,3162 3222,3269 3270,3460 2314,2372 3163,3221 3461,3638 | feature/cds CDS feature/misc sequence_feature feature/stop stop_codon feature/misc sequence_feature ribosome_entry_site feature/rbs CDS feature/cds stem_loop feature/stem_loop stop_codon feature/stop sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter |