Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alaina Shumate
Date created: 2013-10-25 11:00:00
Date modified: 2015-05-08 01:14:03
Myoglobin Actuator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36122_sequence (Version 1) |
Description
Our part takes as input the PoPS signal generated by a sensor and the output is human myoglobin protein. The codons have been optimized for expression in e. coli. The protein will be the protein that can bind a heme group and oxygen to serve its purpose as an oxygen carrier in muscular and skeletal tissuesNotes
We chose to use a strong bicistronic device as the myoglobin protein has not been shown to be hazardous to e coli.The strength was picked based on information in "Precise and reliable gene expression via standard transcription and translation initiation elements". We also chose a strong terminator(BBa_B1006) because we have no need for a leaky terminator. We also had to optimize the codons for use in k12 e. coli. The strength of the terminator was determined based on the paper "Measurement and modeling of intrinsic transcription terminators"Source
The sequence for this part comes from the human genome. Specifically, this gene is located on chromosome 22.| Access | Instance | Definition |
| public public public | BBa_M36120 BBa_M36121 BBa_B1006 | BBa_M36120 BBa_M36121 BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36120 BBa_M36121 BBa_B1006 | 1,88 97,558 567,605 | BBa_M36120 BBa_M36121 BBa_B1006 |