Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matt Kandath and Eric Kofman
Date created: 2012-06-03 11:00:00
Date modified: 2015-05-08 01:14:03
T7 17.5 Plasmid
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_M36161_sequence (Version 1) |
Description
This plasmid contains a bicistronic translation junction, the T7 17.5 holin coding sequence, and a medium strength transcription terminator (Part BBa_M36292). Our bicistronic translation junction is engineered by the BIOFAB team at biofab.org (Part BBa_J119024), and is utilized in order to promote more efficient translation of our coding sequence. The T7 17.5 coding sequence is taken from published genome of T7 (see below for link), available online. Finally, our transcription terminator is taken from the Registry of Standard Biological Parts and has ???99% efficiency???.Notes
Due to the possible toxic nature of our gene (as it is proposed to cause cell lysis), a low copy number origin of replication was used in order to somewhat control against over expression of our gene.Source
Link for T7 genome: http://www.ncbi.nlm.nih.gov/nuccore/NC_001604.1?report=genbank&from=36344&to=36547| Access | Instance | Definition |
| public public public | BBa_J119024 BBa_M36160 BBa_M36292 | BBa_J119024 BBa_M36160 BBa_M36292 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J119024 BBa_M36160 BBa_M36292 | 1,88 97,297 306,387 | BBa_J119024 BBa_M36160 BBa_M36292 |