ADHAc

BBa_M36203 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36203

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Aliado, Steven Lee, Simon Zheng

Date created: 2011-12-06 12:00:00

Date modified: 2015-05-08 01:14:03

ADH Actuator
input is PoPS and output is ADH enzyme

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_M36203_sequence (Version 1)

Description

This actuator receives an input of polymerases per second from a standard sensor and produces ADH1 (Alcohol Dehydrogenase 1) and ADH2 linked to a 6-histidine tag for any necessary for quantitative assays.

This actuator utilizes a bicistronic RBS to prevent binding of the RBS with any other parts of the coding sequence and to maintain consistency in the transcription rate.

This actuator consists of ADH1, which converts acetaldehyde to ethanol, and ADH2, which converts ethanol to acetaldehyde. ADH1 is activated by the presence of acetaldehyde, while ADH2 is activated by the presence of ethanol.

Notes

(1)Codon optimized for Escherichia coli. (2)6-his tag added.

Source

The ADH sequence in the actuator originally codes for Alcohol Dehydrogenase in Acetobacter pasteurianus. Amino acid sequence for Acetobacter pasteurianus can be found here: http://www.ncbi.nlm.nih.gov/nuccore/NC_013211.1?from=15149

Components

Access	Instance	Definition
public	BBa_M36204	BD14
public	BBa_M36200	ADH
public	BBa_M36202	6HisTag
public	BBa_M36201	RhmTrm

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_M36204	1,85	BD14
BBa_M36200	86,1699	ADH
BBa_M36202	1700,1717	6HisTag
BBa_M36201	1718,2895	RhmTrm

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36203/1

Member of these Collections

• iGEM Parts Registry