Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Designed by Cyonna Holmes, Martha Roman, Terry Xu
Date created: 2011-12-06 12:00:00
Date modified: 2015-05-08 01:14:04
ArsAction: Arsenite Removal Device (input: PoPs Signal, output: removal device)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36222_sequence (Version 1) |
Description
The arsenic removal device is designed to prolong cell survival and decrease the rate of cell death due to arsenite toxicity. Our device does this by removing arsenite from the comprimsed cell using a two part removal method. The first coding sequence, arsA, is an ATPase that localizes to the membrane of the cell and confers energy to ArsB. ArsB uses this energy to form an ion efflux pump designed to drive arsenite out of the cell. This device is to be used as an actuator and it responds to the universal PoPs signal. The genes used are specific to the arsenic family and can only be used to create a pump that removes the ion form of arsenic (arsenite).Notes
In designing this part, we had to take into consideration the form of arsenic we wanted to remove. Removing arsenic from a cell called for the insertion of another coding sequence that coded for the gene, arsC. This addition would have significantly increased the total base pair count of our device. Instead, we decided to have our device remove arsenite. Furthermore, the ars family of genes is usually regulated by the arsR operon, but we did not including a coding sequence for arsR in our design. Because we designed our actuator to respond to the PoPS signal, we decided that inclusion of the arsR operon as a regulatory operon would be redundant. Therefore, we removed it.Also, it was necessary to codon optimize each part of our device in order to ensure successful transformation and use in the K-12 strain of E. Coli.
Source
asfasdfasdfas| Access | Instance | Definition |
| public public public public public | BBa_B0034 BBa_M36220 BBa_B0035 BBa_M36221 BBa_B1002 | BBa_B0034 arsA BBa_B0035 arsB BBa_B1002 |