Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lawerence Xing and Andres Gaeta
Date created: 2011-12-10 12:00:00
Date modified: 2015-05-08 01:14:04
Epinephrine sensor construct
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_M36239_sequence (Version 1) |
Description
This part is designed to sense epinephrine, and generate a PoPS signal in response.The QseB and QseC genes are connected to a constitutive promoter, and so will be constantly expressing the QseB and QseC proteins, which will creates the adrenergic receptors (QseC) on the surface of the cell as well as the signaling molecules activated when the receptor is activated (QseB).
When epinephrine is detected by QseC, it autophosphorylates, which in turn will phosphorylate QseB. This, in turn, will bind to the flhDC promoter, activating it. This will cause a PoPS signal to be produced, which can be used with an actuator.
It is important to note that QseC may be activated not only by epinephrine, but also norepinephrine and Auto-Inducer 3 (AI3).
Notes
In order to ensure as high a PoPS signal as possible, we used the strongest promoters, RBS, and transcriptional terminators available.Source
The promoters, bicistronic RBS, and transcriptional terminators were all sourced from BioFab.The QseB and QSeC coding sequences were taken from, EcoCyc, which documented the entire genome of E. Coli.
The flhDC was also taken from EcoCyc's website.
| Access | Instance | Definition |
| public public public public public public public public public | BBa_M36230 BBa_M36236 BBa_M36231 BBa_M36232 BBa_M36233 BBa_M36237 BBa_M36234 BBa_M36235 BBa_K554001 | BBa_M36230 BBa_M36236 BBa_M36231 BBa_M36232 BBa_M36233 BBa_M36237 BBa_M36234 BBa_M36235 flhDC |