BBa_M36532

BBa_M36532 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36532
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Inderpreet Kaur Hayer
Date created: 2015-10-23 11:00:00
Date modified: 2015-10-24 08:01:43

Arsenate Bioremediation Actuator in S. Cerevisiae



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_M36532_sequence (Version 1)

Description

Encodes transport and reduction of arsenate into arsenite. This actuator is based off of naturally occurring contiguous genes in the yeast Saccharomyces cerevisiae which are involved in resistance to arsenic compounds. PHO84 is a phosphate transporter which co-transports arsenate with high efficiency across the cell membrane. In recent studies, overexpression of PHO84 increases arsenate internalization by 60% compared to a wild-type control strain. ACR 1 is required for transcription of genes involved in resistance to arsenic compounds. ACR 2 is the arsenate reductase, converting arsenate to arsenite, which is required for arsenate resistance. This actuator can be attached to galactose inducible/glucose repressible promoters.

Notes

Due to the size of PHO84, it was necessary to design a separate construct with the ACR genes, 1 and 2.

Source

Naturally occurring genes in S. cerevisiae: Pho84, ACR 1 and 2

Access Instance Definition
public
public
BBa_M36533
BBa_M36534
BBa_M36533
BBa_M36534
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36532/1