Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elisa Vidales
Date created: 2015-10-21 11:00:00
Date modified: 2015-10-23 06:17:49
LasR/PAI complex sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36580_sequence (Version 1) |
Description
Sensor for complex made up of LasR bound to Pseudomonas Autoinducer. Contains qsc102 promoter, BBa_0030 strong RBS, GFP coding sequence.Notes
Added preceding spacing to qsc102 promoter palindromic binding sequence, as well as spacing flanking the RBS in order to optimize binding.Source
qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
BBa_0030 strong RBS: partsregistry.org
GFP coding sequence: BIOE44 pComet DNA sequence
| Access | Instance | Definition |
| public public | BBa_M36576 BBa_B0032 | BBa_M36576 BBa_B0032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36576 BBa_B0032 | 1,65 74,86 | BBa_M36576 BBa_B0032 |