Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aris Kare, Eda Benites, Ricky Cordova
Date created: 2015-10-23 11:00:00
Date modified: 2015-12-05 03:14:09
Making acetoacetate from acetic acid and acetoacetyl-CoA
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36584_sequence (Version 1) |
Description
This part begins with a promoter, the ctfA coding sequence, a strong RBS, the ctfB coding sequence, and a termination sequence from T1.Notes
Chose to use only one promoter for both ctfA and ctfB to shorten the length of the plasmid.Source
Sequence for ctfA obtained from http://www.ncbi.nlm.nih.gov/gene/1116168Sequence for ctfB obtained from http://www.ncbi.nlm.nih.gov/gene/1116169
| Access | Instance | Definition |
| public public public public public | BBa_M36933 BBa_M36581 BBa_M36583 BBa_M36582 BBa_M36586 | BBa_M36933 BBa_M36581 BBa_M36583 BBa_M36582 BBa_M36586 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36933 BBa_M36581 BBa_M36583 BBa_M36582 BBa_M36586 | 1,114 115,771 772,799 800,1465 1466,1544 | BBa_M36933 BBa_M36581 BBa_M36583 BBa_M36582 BBa_M36586 |