Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Hu, Andrew Austin Lee, Clinton Olivas
Date created: 2014-10-23 11:00:00
Date modified: 2015-05-08 01:14:05
Yeast-Optimized Chicken Lysozyme (LysC) Secretor
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M36598_sequence (Version 1) |
Description
Our device can be transformed into yeast for the production of mammalian lysozyme. It consists of a GAL1 promoter, the LysC gene linked to a yeast-optimized albumin signal peptide, and a Cyc1 terminator. Chicken lysozyme (LysC) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.Notes
We checked to make sure the sequence did not include any BsaI restriction sites because we are cloning the gene into a vector using BsaI. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized.Source
The Lysozyme sequence is originally derived from Gallus Gallus. The Gal1 promoter and Cyc1 terminator are originally derived from Saccharomyces cerevisiae.| Access | Instance | Definition |
| public public public | BBa_J63006 BBa_M36762 BBa_K1462070 | GAL1 BBa_M36762 BBa_K1462070 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J63006 BBa_M36762 BBa_K1462070 | 1,549 556,999 1008,1257 | GAL1 BBa_M36762 BBa_K1462070 |