Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz
Date created: 2015-10-22 11:00:00
Date modified: 2015-12-04 06:55:33
Poly-3-hydroxybutyrate Growth Actuator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36659_sequence (Version 1) |
Description
Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. This composite part is simply the DNA sequence collected from Xanthomonas campestris pv. raphani 756C, specifically from the gene G0CFI5.Notes
Design considerations include limiting the DNA sequence to 1500 bp, avoiding BbsI, BsaI, and BsmBI, and ensuring less than five repeats of 10 bp. Restriction sites were avoided to not conflict with DNA 2.0 synthesis process, as was the minimization of repeats in the sequence.Source
Xanthomonas campestris pv. raphani 756C is the organism from which the DNA sequence for poly-beta-hydroxybutyrate polymerase.References include http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en.
| Access | Instance | Definition |
| public public | BBa_M36660 BBa_M36658 | BBa_M36660 BBa_M36658 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36660 BBa_M36658 | 1,1077 1084,1806 | BBa_M36660 BBa_M36658 |