Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ian Hull, Poorwa Godbole
Date created: 2014-04-29 11:00:00
Date modified: 2015-05-08 01:14:05
HeLa beta-galactosidase actuator with secretion tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36666_sequence (Version 1) |
Description
This actuator is designed to produce beta-galactosidase with an N-terminal secretion tag in HeLa cells. It links the alpha and omega fragments of the E. coli lacZ gene, though the codons have been optimized for expression in HeLa cells. It includes the kozak sequence immediately before the start codon and a secretion tag immediately after the start codon.Notes
The lacZ alpha fragment and lacZ omega fragment were both codon optimized for expression in HeLa cells. After combining all the parts, there were only 7 repeats of ≥10 base pairs, so in order to maintain codon optimization, no further changes were made.Source
Kozak sequence obtained from Part:BBa_J63003. Secretion tag obtained from Part:BBa_K782062. LacZ alpha fragment obtained from Part:BBa_E0051. LacZ omega fragment obtained from Part:BBa_K891003. Poly-A tail obtained from Part:BBa_M36096.| Sequence Annotation | Location | Component / Role(s) |
| Kozak sequence start codon PPT secretion tag HeLa-optimized beta-galactosidase lacZ alpha fragment lacZ omega fragment poly-A tail | 1,12 13,15 16,57 58,1101 58,237 238,1101 1102,1227 | feature/misc sequence_feature start_codon feature/start feature/tag tag CDS feature/protein CDS feature/cds CDS feature/cds polyA_site feature/polya |