Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ray Paul Laureano-Mercado & Mark Kirollos
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:06
PoPS -> Melamine deaminase
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_M36782_sequence (Version 1) |
Description
This actuator's purpose is to code for the output of the enzyme melamine deaminase. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations. Its design comprises three components, which include a bicistronic ribosome binding site, the MDA gene of interest which codes from melamine deaminase, and a transcription terminator. The part needs a PoPS signal as an input in order to produce the desired enzyme and was designed to work in E. coli.Notes
The TriA gene???s codons were modified from the original sequence and optimized for E. coli to a Codon Adaptation Index (CAI) of .76 (Puigb??, P., Guzm??n, E., Romeu, A. & Garcia-Vallv??, S. OPTIMIZER: A web server for optimizing the codon usage of DNA sequences. Nucleic Acids Research. 2007;35:W126???31.). After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis. The sequence shown maintains a CAI of .76 and the same amino acid sequence as the original TriA gene.Source
The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744). The sequences for the RBS and Terminator were sourced from BIOFAB (http://biofab.org/)| Access | Instance | Definition |
| public public public | BBa_M36236 BBa_M36761 BBa_B1002 | BBa_M36236 MDA BBa_B1002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36236 BBa_M36761 BBa_B1002 | 1,85 86,1510 1511,1544 | BBa_M36236 MDA BBa_B1002 |