BBa_M36916

BBa_M36916 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36916
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rohit Talreja, Kelly Nguyen, Jacqueline Tao
Date created: 2012-12-06 12:00:00
Date modified: 2015-05-08 01:14:07

This series of parts codes for the expression of the benzylalcohol acetyltransferase enzyme



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_M36916_sequence (Version 1)

Description

This is a coding sequence consisting of the:
BBa_M36913: bicistronic RBS
BBa_M36915: coding DNA sequence for the benzylalcohol acetyltransferase (BEAT) enzyme. This enzyme converts benzyl alcohol into benzylacetate (a volatile, odorous compound excreted from the petals of many species of flowers).
BBa_M36912: transcription terminator

Notes

Needed to optimize part for several strengths of PoPS input and expression in E. coli

Source

The terminator and bicistronic RBS come from the BIOFAB standards part database
Mutalik, Vivek, et al. ???Rules for Precision Gene Expression via Reusable Standard Biological Parts???

The coding DNA sequence was extracted by Natalia Dudareva et al
Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran
Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral
Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36916/1