BBa_M36980

BBa_M36980 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_M36980
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nick Davis, Rachel RoseFigura
Date created: 2011-05-02 11:00:00
Date modified: 2015-05-08 01:14:07

Coenzyme B-12 Biosensor for E. coli (



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_M36980_sequence (Version 1)

Description

Composite part for a Vitamin B12 Biosensor for Escherichia coli. Features a constitutively-active promoter (~99% promotion efficiency), the naturally-occurring vitamin B-12 riboswitch sequence, gemini fusion protein coding sequence (coding for both GFP and ??-galactosidase production), and a strong promoter (~99% termination efficiency).

Notes

As opposed to more "traditional" biological sensor and actuator parts (that operate on the principle of PoPs), this composite Vit B12 Biosensor relies on RiPS (Ribosomes per second), as the main sensing structure (part M36998) is the naturally-occurring vitamin B12 riboswitch???a length of RNA that forms complex aptamer structures upon binding of vitamin B12 as the substrate.

Source

Designed by Nick Davis and Rachel RoseFigura, Bioengineering undergraduate students at Stanford University.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36980/1