BBa_M36983

BBa_M36983 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36983

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathan Barnett, Meghan Bowler, Kian Torabian

Date created: 2011-05-16 11:00:00

Date modified: 2015-05-08 01:14:07

Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_M36983_sequence (Version 1)

Description

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Notes

This composite part consists of a strong 5' UTR Sequence, followed by a signal peptide for secretion from E.coli, the non-glycolated AFP 5-mer, and finally a strong termination sequence. The 5' UTR and termination sequences are very strong because we want high transcription and only transcription of the region we coded before the termination sequence. The signal peptide is N-terminal and is native to E.coli.

Source

Pseudopleuronecta americanus (White Flounder) and E. Coli

Components

Access	Instance	Definition
public	BBa_M36009	BBa_M36009
public	BBa_M36981	BBa_M36981
public	BBa_M36999	BBa_M36999
public	BBa_M36010	BBa_M36010

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_M36009	1,47	BBa_M36009
BBa_M36981	48,119	BBa_M36981
BBa_M36999	120,320	BBa_M36999
BBa_M36010	321,402	BBa_M36010

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36983/1

Member of these Collections

• iGEM Parts Registry