BBa_M36983

BBa_M36983 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36983
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-05-16 11:00:00
Date modified: 2015-05-08 01:14:07

Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_M36983_sequence (Version 1)

Description

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Notes

This composite part consists of a strong 5' UTR Sequence, followed by a signal peptide for secretion from E.coli, the non-glycolated AFP 5-mer, and finally a strong termination sequence. The 5' UTR and termination sequences are very strong because we want high transcription and only transcription of the region we coded before the termination sequence. The signal peptide is N-terminal and is native to E.coli.

Source

Pseudopleuronecta americanus (White Flounder) and E. Coli

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36983/1