Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Federico C. Rodriguez
Date created: 2014-04-18 11:00:00
Date modified: 2015-05-08 01:14:08
Uranium Inducible GFPuv Reporter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_M45117_sequence (Version 1) |
Description
This composite part is made out of the uranium inducible (urcA)promoter (BBa_M45114), a ribosome binding site, the GFPuv protein which is a green fluorescent protein (GFP) variant optimized for excitation by UV light, and an extra stop codon to prevent RNA polymerase from reading it through.Notes
The complete sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.Source
This composite part comes from BBa_M45113 + BBa_B0034 + BBa_M45116 + BBa_B0010 + BBa_B0012.| Access | Instance | Definition |
| public public public public public | BBa_M45113 BBa_B0034 BBa_M45116 BBa_B0010 BBa_B0012 | BBa_M45113 BBa_B0034 BBa_M45116 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M45113 BBa_B0034 BBa_M45116 BBa_B0010 BBa_B0012 | 1,974 983,994 1001,1717 1726,1805 1814,1854 | BBa_M45113 BBa_B0034 BBa_M45116 BBa_B0010 BBa_B0012 |