Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sherissa Ward
Date created: 2014-04-20 11:00:00
Date modified: 2015-05-08 01:14:08
F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M45125_sequence (Version 1) |
Description
OriT is the nic region on the F Plasmid that allows for conjugation in the presence of transfer genes encoded in TraJ genes. This OriT gene is followed by an autolysin N-acetylmuramoyl-L-alanine amidase. This codes for an Autolysin that will cause cell death to the cell upon conjugation. This composite part is meant to be incorporated into a plasmid with the TraJ genes knocked out with a kanamycin resistance gene. This will further prevent conjugation by the bacteria.Notes
There may need to be up-regulation of the autolysin in this case to increase the rate of cell death.Source
This part was made using an autolysin from Bacillus subtilis and other parts in the registry.| Access | Instance | Definition |
| public public public public | BBa_J01002 BBa_J54200 BBa_B0030 BBa_M45122 | OriTF BBa_J54200 BBa_B0030 BBa_M45122 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J01002 BBa_J54200 BBa_B0030 BBa_M45122 | 1,365 374,423 432,446 453,1940 | OriTF BBa_J54200 BBa_B0030 BBa_M45122 |