Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cale Lester, Raquel Freeman, Tristan Yeung
Date created: 2016-11-01 12:00:00
Date modified: 2016-12-11 07:37:42
U1_mTurquoise2, IPTG-induced, CAM resistant Thermosensor Construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M50033_sequence (Version 1) |
Description
At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. U1 has a unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. It contains a pP-T5 promoter, U1 RBS, mTurquoise2, and a pT-T7 terminator.Notes
No BsaI restriction sites. Under 2kb for synthesis using DNA 2.0.Source
Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.
| Access | Instance | Definition |
| public public public public | BBa_M50031 BBa_M50007 BBa_M50029 BBa_M50032 | BBa_M50031 BBa_M50007 BBa_M50029 BBa_M50032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M50031 BBa_M50007 BBa_M50029 BBa_M50032 | 1,48 49,86 87,806 807,854 | BBa_M50031 BBa_M50007 BBa_M50029 BBa_M50032 |