Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cale Lester, Raquel Freeman, Tristan Yeung
Date created: 2016-11-01 12:00:00
Date modified: 2016-12-11 08:10:23
U10_mKO2, IPTG inducible, KAN resistant Thermosensor Construct
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M50034_sequence (Version 1) |
Description
At elevated temperatures, the U10 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. The U10 thermosensor mainly exhibits activity at approximately 37˚C. Other temperatures such as 22˚C and 30˚C show very low gene expression levels. We picked U10 because of its higher specificity as a thermosensor in our chosen temperature ranges (22˚C - 37˚C). We did not make any modifications to the thermosensor genetic sequences given by Neupert, J., Karcher, D., and Bock, R.Notes
Removed 2 BsaI restriction sites. Under 2 kb for synthesis by DNA 2.0.Source
Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.
| Access | Instance | Definition |
| public public public public | BBa_M50031 BBa_M50008 BBa_M50030 BBa_M50032 | BBa_M50031 BBa_M50008 BBa_M50030 BBa_M50032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M50031 BBa_M50008 BBa_M50030 BBa_M50032 | 1,48 49,87 88,744 745,792 | BBa_M50031 BBa_M50008 BBa_M50030 BBa_M50032 |