Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Agbay
Date created: 2016-12-11 12:00:00
Date modified: 2016-12-12 01:31:50
IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_M50050_sequence (Version 1) |
Description
When transfected into E. coli, this plasmid will augment it's ability to express the LuxS synthase protein, allowing for differing production of Autoinducer-2. The expression of this plasmid is controlled by inducing with IPTG, allowing for artificial control of increased AI-2 levels.Notes
Designing this part required no modifications.Source
The source of the LuxS gene came from UniProt. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH.| Access | Instance | Definition |
| public public public public public | BBa_K592008 BBa_J61100 BBa_M50027 BBa_K133133 BBa_B0010 | PT5lac BBa_J61100 BBa_M50027 His-Stop BBa_B0010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592008 BBa_J61100 BBa_M50027 BBa_K133133 BBa_B0010 | 1,126 135,146 153,671 680,700 709,788 | PT5lac BBa_J61100 BBa_M50027 His-Stop BBa_B0010 |