Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Agbay
Date created: 2016-12-11 12:00:00
Date modified: 2016-12-11 03:30:39
IPTG Inducible Plasmid for the Expression of LsrR Repressor
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_M50051_sequence (Version 1) |
Description
This plasmid augments the natural expression of the LsrR repressor for the lsr operon. It is controlled through the induction of IPTG, allowing for close control of artificial expression.Notes
We did not run into any major design considerations after analyzing our construct using Gene Designer.Source
The genetic sequence of the LsrR gene was sourced through the UniProt database. Other parts were sourced through DNA 2.0 under the plasmid name pD444-CH.| Access | Instance | Definition |
| public public public public public | BBa_K592008 BBa_J61100 BBa_M50028 BBa_K133133 BBa_B0010 | PT5lac BBa_J61100 BBa_M50028 His-Stop BBa_B0010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592008 BBa_J61100 BBa_M50028 BBa_K133133 BBa_B0010 | 1,126 135,146 153,1109 1118,1138 1147,1226 | PT5lac BBa_J61100 BBa_M50028 His-Stop BBa_B0010 |